July 4, 2016

The cluster of differentiation (cluster of designation or classification determinant) (often abbreviated as CD) is a protocol used for the identification and investigation of cell surface molecules providing targets for immuno-phenotyping of cells. Nomenclature propose...

February 6, 2016

The accuracy and quality of the data is the major factor in  using multiple fluorochromes in an experiment. The most critical factor is determining which color should be matched to each antibody in the reagent panel. This is due to a very large range of intrinsic brigh...

November 23, 2015

Neural cell adhesion molecule (NCAM), also called CD56, is a homophilic binding glycoprotein expressed on the surface of neurons, glia, skeletal muscle and natural killer cells. NCAM has been implicated as having a role in cell–cell adhesion,neurite outgrowth, synaptic...

November 7, 2015

CD3 is initially expressed in the cytoplasm of pro-thymocytes, the stem cells from which T-cells arise in the thymus. The pro-thymocytes differentiate into common thymocytes, and then into medullary thymocytes, and it is at this latter stage that CD3 antigen begins to...

October 25, 2015

White blood cells are like the bouncers of our bodies -- they look for potentially harmful, invading parties and try to give them the boot. Basophils are a particular type of bouncer who belong to the group of white blood cells known as the granulocytes. The granulocyt...

October 9, 2015

Flow cytometers in general are not made to provide precise volumetric counting (rather relative percentages between particles in the same sample). For "absolute counts", you have to add counting beads like Flow-Count beads(10 µm from Beckman-Coulter), Tru-Count beads p...

October 6, 2015

 

Theoretically  any type of sample can be used for flow cytometry as all that is required is a single cell suspension of intact cellular structures. We routinely run various immune preparations, and they can be performed both fresh  or  fixed (when performing immunophe...

October 4, 2015

Stain index is a measure of resolution sensitivity (the ability to resolve a dim positive signal from background).   It depends upon the difference between positive and background peak medians (D) and the spread of the background peak (W).  The stain index is a metric...

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