DATA VARIATION ON DIFFERENT SPEED OF FLOWCYTOMETRY:

October 9, 2015

Flow cytometers in general are not made to provide precise volumetric counting (rather relative percentages between particles in the same sample). For "absolute counts", you have to add counting beads like Flow-Count beads(10 µm from Beckman-Coulter), Tru-Count beads provided in tubes (~4.5 µm from Becton-Dickinson), Cyto-Count (~6µm beads from DAKO),  and Perfect-Count Microspheres (~6µm beads from Cytognos, Spain). All those are made for counting cells in suspension. If you increase the flow rate you increase the introduction of particles through the laser. Your counts should theoretically be proportional to the speed at each level (LOW - MED - HIGH).  more

 

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