Acute monoblastic and acute monocytic leukemia (AML- M5)

Acute monoblastic leukemia (AML-M5), is one of the most common subtypes of acute myeloid leukemia  that is either comprised of more than 80% of monoblasts (AML-M5a) or 30-80% monoblasts with (pro)monocytic differentiation (AML-M5b).

Immunophenotype:

At least two monocytic markers (CD4, CD14, CD11b, CD11c , CD36, CD64, CD68 and HLA-DR), Myeloid markers:( CD13 , CD33 , CD15 and CD65) and Abberant expression (CD7, CD56 (25 - 40%), Lysozyme, CD68 and CD163) with CD34 (30%), CD117 and MPO (more often M5b and less often M5a).

Example:

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Above case shows (lymp-green, grans-blue, monoblast-red) positivity for CD33, CD15dim, CD65, CD11b, CD64, CD9dim, CD4, CD56, CD36dim  and negativity for CD7, CD19, HLA-DR ,CD15,CD117, CD13,  cCD79a, cCD3, CD2

References

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          Vol 5,Issue 3,

                  2020

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