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Real time or Live gate:

A gate is a numerical or graphical boundary that can be used to define the characteristics of particles to include for further analysis. The gating process is simply selecting an area on the scatter or histogram plot generated during the flow experiment that decides which cells you want to continue to analyze.

A live gate is a term used for a process that prevents the acquisition by the computer of non-selected data from the flow cytometer.

 

Figure 1. Gating shows normal gate and live gate  

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