top of page

OPTICAL SYSTEM:

  • The optical system is comprised of light excitation source (lasers) of a specific wavelength, and optical filters and mirrors which direct specific wavelengths of light to their appropriate detector.

  • Flow cytometers have three or more lasers of different wavelengths. This provides for multiple fluorescent measurements simultaneously.

  • Several optical filters are arranged in front of each photomultiplier tube (PMT) to direct the desired wavelength of light to the detector.

  • Photodiodes are commonly used for strong signals, such as forward light scatter.

  • Both detectors convert light signal into an electrical signal that can be further processed and analyzed by computers

       Components of Optics 

Laser

            Optical filters

     Detectors

OPTICAL SYSTEM LAYOUT

 

bottom of page