

FlowCytometry Net
(Flow Cytometry Based Knowledge Portal)
OPTICAL SYSTEM:
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The optical system is comprised of light excitation source (lasers) of a specific wavelength, and optical filters and mirrors which direct specific wavelengths of light to their appropriate detector.
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Flow cytometers have three or more lasers of different wavelengths. This provides for multiple fluorescent measurements simultaneously.
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Several optical filters are arranged in front of each photomultiplier tube (PMT) to direct the desired wavelength of light to the detector.
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Photodiodes are commonly used for strong signals, such as forward light scatter.
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Both detectors convert light signal into an electrical signal that can be further processed and analyzed by computers
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Components of Optics
Laser
Optical filters
Detectors
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OPTICAL SYSTEM LAYOUT
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