FlowCytometry Net
(Flow Cytometry Based Knowledge Portal)
Reagents for flow cytometry
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PBS 10 X (Stock)
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PBSA 1 X (Working)
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Lysing solution 10 X & 1 X
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PBS-PFA
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1 liter stock of 10x PBS can be prepared by dissolving
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7.2 g NaCl,
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1.32 g Na2HPO4 · 2H2O
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7.52 g KH2PO4
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Make upto 1 L by distilled water.
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pH 7.2 ± 0.2, Store 2-80C in tightly closed bottle
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Dil stock 1 in 10 to a total vol that you would consume in a weak for 1x PBS working
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1 liter stock of 1x PBSA can be prepared by dissolving
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10g BSA
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2.5g Sodium Azide
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Make upto 1 L by 1x PBS.
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pH 7.2 ± 0.2, Store 2-80C in tightly closed bottle
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1 litre of Lysing solution stock (10X)
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89.9g NH4Cl
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10.0g KHCO3
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3.7g EDTA Na4
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DW to 1 lit.
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pH 7.2 ± 0.2, Store 2-80C in tightly closed bottle
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100ml Lysing solution working (1X)
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90 ml DW + 10 ml 10X stock
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Mix well. Store at RT. Discard after 3 days
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PBS – PFA (0.5%)
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PFA 0.5g in 100 ml of 1 X PBS (with azide)
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Dissolve by heating to 65-700C (hot plate with magnetic stirrer is best). Must keep flask/beaker covered tightly with AL foil while heating.
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Filter solution though a 0.2 μ suction filter before use esp for minimal residual disease work.
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Store at 40C for upto 2 weaks