SYTO Dye:

 

Green-fluorescent nucleic acid stains are cell-permeant nucleic acid stains that show a large fluorescence enhancement upon binding nucleic acids. The SYTO dyes can be used to stain RNA and DNA in both live and dead eukaryotic cells, as well as in Gram-positive and Gram-negative bacteria. Available as blue-, green-, orange- or red-fluorescent dyes, these novel SYTO stains share several important characteristics:

• Permeability to virtually all cell membranes, including mammalian cells and bacteria

• High molar absorptivity, with extinction coefficients >50,000 cm-1M-1at visible absorption maxima

• Extremely low intrinsic fluorescence, with quantum yields typically <0.01 when not bound to nucleic acids

• Quantum yields that are typically >0.4 when bound to nucleic acid

 

 The use of SYTO-13, a member of the class of SYTO dyes, as a new indicator of viability. The SYTO dyes, among which is the most popular SYTO-13, are cell-permeant nucleic acid stains that permit myriad of applications such as the discrimination between live/dead eukaryotic or prokaryotic cells, the detection of apoptosis, or germinated bacterial endospores.The cell-permeant SYTO 13 green fluorescent nucleic acid stain exhibits bright, green fluorescence upon binding to nucleic acids.

 

A fifty μl aliquot of the MP suspension was diluted to 400 μl (1:8) in PBS or in 200 nM solution of SYTO 13. The 200 nM SYTO 13 solution was prepared by diluting 1 μl of 5 mM SYTO 13 in 25 ml of PBS.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

                                                       

 

 

 

 

 

 

 

 

                                                               Above is the two example of the Syto 13 dye.  both figures shows the red (live) and green(dead) population.

REFERENCE:

 

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